邻近编码技术

突破性的单外泌体检测技术，改变了传统外泌体技术往往局限于总蛋白、总核酸检测的困境，实现了高精度、高灵敏度的外泌体异质性研究。

邻近编码技术（Proximity Barcoding Assay，PBA）文章：Wu et al. (2019) Profiling surface proteins on individual exosomes using a proximity barcoding assay

样本中单个外泌体的表面蛋白组成通过与之结合的抗体-DNA偶联物可被转化为DNA序列信息。

流程步骤

1、抗体-DNA偶联物识别外泌体表面蛋白并结合，PBA技术可以实现同时对单外泌体表面200+蛋白进行识别标记。

2、滚环DNA序列通过识别单外泌体表面标记物，实现单外泌体分隔并标记。

3、新一代测序技术解析DNA序列中的蛋白与外泌体信息。

4、生物信息学分析单外泌体蛋白组学信息。

创新与优势

1、高通量单外泌体（10万）分析

对于每个样本中10万+的单外泌体蛋白进行同步分析，为您发现样本中低丰度外泌体亚群。

2、<5ul样品量且无需提纯

绝大部分仅仅需要微量样本，无需预先进行外泌体分离纯化步骤，进一步节约时间与实验成本。

3、单信号分析检测高灵敏度的数据结果，检测分辨率精确至单外泌体表面分子蛋白，可识别具有更高疾病诊断价值的低丰度外泌体表面蛋白。

4、适用于几乎所有体液、培养液

例如血浆、尿液、细胞培养液、器官灌洗液均能进行单外泌体检测，适用样本类型广泛，可以实现更多不同种类样本的单外泌体检测，大大减少了样本的各类限制。

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