甲状腺乳头状癌piRNA诊断标志

PIWI-interacting RNAs piR-13643 and piR-21238 Are Promising Diagnostic Biomarkers of Papillary Thyroid Carcinoma5.515Aging (Albany NY) . 2020 May 19;12(10):9292-9310. doi: 10.18632/aging.103206. Epub 2020 May 19.

Abstract

Emerging studies demonstrate that PIWI-interacting RNAs (piRNAs) participate in the development of cancers. 75 pairs of papillary thyroid carcinoma (PTC) samples and 31 benign thyroid nodule samples were included in this three-phase biomarker identifying study. First, piRNA expression profiles of five pairs of PTC samples were acquired piRNA sequencing. The expression of all upregulated piRNAs were further validated by RT-qPCR. Paired t and nonparametric test were used to evaluate the association between all upregulated piRNAs and clinic stage. The expression levels of key piRNAs were corrected by demographic data to construct a multivariate model to distinguish malignant nodules from benign. Additionally, the intersection between target genes of key piRNAs and differentially expressed genes in The Cancer Genome Atlas (TCGA) PTC samples were used to perform enrichment analysis. Only piR-13643 and piR-21238 were significantly upregulated in PTC and associated with clinic stage. Moreover, both piR-13643 (Area Under Curve (AUC): 0.821) and piR-21238 (AUC: 0.823) showed better performance in distinguishing malignant nodules from benign than currently used biomarkers HBME1 (AUC: 0.590). Based on our findings, piR-13643 and piR-21238 were observed to be significantly upregulated in human PTC. PIWI-interacting RNAs could serve as promising novel biomarkers for accurate detection of PTC.  

Keywords: PIWI-interacting RNAs; biomarkers; diagnosis; nomogram; thyroid cancer.

今天跟大家分享的是2020年5月中旬发表在AGING-US （IF: 5.515）杂志上的一篇识别关键piRNAs作为甲状腺乳头状癌（PTC）诊断标志的文章。想必很多同学对piRNAs都不是很熟悉吧，那么到底什么是piRNAs，我们又该如何通过常用的生物信息学方法识别出可以作为标志物的关键piRNAs呢？就让我们带着这样的疑问开始今天的学习吧。